說明書下載DATG.rar

分類名稱 : 高效藥物篩選,酶活性測定 產(chǎn)品介紹 :

產(chǎn)品說明  ATP酶和GTP酶催化ATP或GTP分解為ADP或GDP和磷酸鹽離子。這些酶在能量運輸、信號傳導(dǎo)、蛋白質(zhì)合成和細(xì)胞分化中起著關(guān)鍵的作用。本公司研制的ATP酶/GTP酶檢測試劑盒采用微板方式，為ATP酶/GTP酶活性檢測提供了一個靈敏的手段。它能在30分鐘內(nèi)準(zhǔn)確檢測出酶的活性。其測試方法是利用改良試劑與被釋放的磷酸鹽離子形成深綠色復(fù)合物的特性，用孔板吸光率閱讀儀在波長600-660nm處檢測。

產(chǎn)品特點   測量敏感、準(zhǔn)確：能檢測2 pmole的磷酸鹽離子?？焖?、簡便：單一試劑，可以在30分鐘內(nèi)對酶活性進(jìn)行定量。高通量：在波長620nm處檢測能夠*地減少有色化合物的干擾；在96孔板和384孔板檢測中，Z’因子大于0.7；可用于高通量自動化檢測。

產(chǎn)品用途  1.用于ATP酶和GTP酶活性的測定。2. 用于藥物開發(fā)和高通量篩選ATP酶或GTP酶抑制劑。

儲藏條件  在4°C環(huán)境下，試劑和標(biāo)準(zhǔn)品能保存1年。

DESCRIPTION  ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation. Our ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).

KEY FEATURES  High sensitivity: detection of as little of 2 pmoles of free phosphate. Fast and convenient: single reagent, homogeneous “mix-and-measure” assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z’ factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

APPLICATIONS  1. Determination of ATPase and GTPase activity. 2. Drug Discovery: high-throughput screen for ATPase or GTPase inhibitors.

STORAGE CONDITIONS  The reagents and standard are stable for one year when stored at 4°C.

其他 :

編號：DATG-048，DATG-200

測試：48，200T