Qiagen 74134 細(xì)胞組織RNA提取試劑盒
操作步驟:應(yīng)用QIAGEN Plasmid Kits純化過(guò)程中,通過(guò)離心取出細(xì)菌裂解液。然后將澄清的裂解液裝入陰離子交換柱,在適當(dāng)?shù)牡望}濃度和pH值條件下,陰離子交換柱會(huì)選擇性地結(jié)合質(zhì)粒DNA。RNA、蛋白質(zhì)、代謝物和其他小分子雜質(zhì)通過(guò)中鹽濃度洗滌去除,應(yīng)用高鹽濃度緩沖夜洗脫超純的質(zhì)粒DNA(見(jiàn)流程圖"Comparison of QIAGEN Plasmid Kit Procedures")。通過(guò)異丙醇沉淀濃縮并脫鹽DNA,離心法收集DNA。
Qiagen 12243 | QIAfilter Plasmid Midi Kit (25) | 快速純化轉(zhuǎn)染級(jí)質(zhì)粒試劑盒 |
Qiagen 12362 | EndoFree Plasmid Maxi Kit (10) | 質(zhì)粒大提試劑盒 |
Qiagen 12462 | QIAGEN Large-Construct Kit (10) | DNA質(zhì)粒純化 |
Qiagen 20021 | QIAEX II Gel Extraction Kit (150) | 膠回收純化DNA |
Qiagen 27104 | QIAprep Spin Miniprep Kit (50) | 提取高純度質(zhì)粒DNA |
Qiagen 27106 | QIAprep Spin Miniprep Kit (250) | 提取高純度質(zhì)粒DNA |
Qiagen 28004 | MinElute PCR Purification Kit (50) | 微量PCR回收試劑盒 |
Qiagen 28104 | QIAquick PCR Purification Kit (50) | PCR回收試劑盒 |
Qiagen 28106 | QIAquick PCR Purification Kit (250) | PCR回收試劑盒 |
Qiagen 28306 | QIAquick Nucleotide Removal Kit (250) | 從酶促反應(yīng)物中回收純化 |
Qiagen 28304 | QIAquick Nucleotide Removal Kit (50) | 從酶促反應(yīng)物中回收純化 |
Qiagen 28704 | QIAquick Gel Extraction Kit (50) | 膠回收試劑盒 |
Qiagen 28706 | QIAquick Gel Extraction Kit (250) | 膠回收試劑盒 |
Qiagen 30210 | Ni-NTA Agarose (25 ml) | 6His蛋白純化柱料 |
Qiagen 51104 | QIAamp DNA Blood Mini Kit (50) | 血液DNA提取試劑盒 |
Qiagen 51106 | QIAamp DNA Blood Mini Kit (250) | 血液DNA提取試劑盒 |
Qiagen 51183 | QIAamp DNA Blood Midi Kit (20) | 血液DNA提取試劑盒 |
Qiagen 51185 | QIAamp DNA Blood Midi Kit (100) | 血液中提試劑盒 |