wako 125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase
【產(chǎn)品詳情】
賴氨酰肽鏈內(nèi)切酶
品牌:WAKO 和光
級(jí)別:質(zhì)譜級(jí)
wako 125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase
英文品名:Lysyl Endopeptidase?, Mass Spectrometry Grade
貨號(hào):125-05061
規(guī)格:5×20 μg
應(yīng)用:蛋白質(zhì)組學(xué)。
用途:賴氨酰肽鏈內(nèi)切酶,蛋白測序、質(zhì)譜分析,多肽合成
wako 125-05061賴氨酰肽鏈內(nèi)切酶 Lysyl Endopeptidase
【實(shí)驗(yàn)原理】
賴氨酰肽鏈內(nèi)切酶是質(zhì)譜分析前處理時(shí)常用的蛋白分解酶即賴氨酰肽鏈內(nèi)切酶,賴氨酰肽鏈內(nèi)切酶可以特異性切除賴氨酸基團(tuán)C 末端的多肽,賴氨酰肽鏈內(nèi)切酶可用于蛋白測序分析和Lys-X 化合物的酶合成。
若同時(shí)使用賴氨酰肽鏈內(nèi)切酶和胰酶,可更好地切斷賴氨酸基團(tuán)的多肽,增加多肽的數(shù)量。賴氨酰肽鏈內(nèi)切酶已按照使用習(xí)慣做成小包裝,是方便使用賴氨酰肽鏈內(nèi)切酶的冷凍干燥品。
【產(chǎn)品特點(diǎn)】
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
1)賴氨酰肽鏈內(nèi)切酶用于蛋白質(zhì)譜分析:高特異性、高蛋白質(zhì)消化率;
2)賴氨酰肽鏈內(nèi)切酶能增加肽段數(shù)量、提高裂解效率;
3)賴氨酰肽鏈內(nèi)切酶獨(dú)立包裝,20 μg/一支
【結(jié)果】表明Lep 酶解的錯(cuò)誤裂解率低。
Tp 酶解時(shí)加入Lep 后,錯(cuò)誤裂解率有所降低,同時(shí),賴氨酰肽鏈內(nèi)切酶可以鑒定出更多的多肽。
Tp | Lep | Lep +Tp | |
裂解位點(diǎn) | 精氨酸和賴氨酸的C 端 | 賴氨酸的C端 | 精氨酸和賴氨酸的C端 |
錯(cuò)誤裂解率( 錯(cuò)誤裂解所占比例) | 多(8 %) | 很少(0 %) | 少(3 %) |
鑒定出的多肽數(shù)量 | 17 | 19 | 22 |
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
Lep+Tp 賴氨酰肽鏈內(nèi)切酶切后,可以在m/z=2000 時(shí)得到吸收峰,而單獨(dú)的Tp 酶切在m/z=2000 時(shí)沒有吸收峰。該結(jié)果表明Lep 可以提高測序覆蓋度。
賴氨酰肽鏈內(nèi)切酶Lysyl Endopeptidase 125-05061 wako
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