K-FRUCL D-果糖/D-葡萄糖檢測試劑盒

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

品牌：Megazyme

貨號：K-GLUCL

中文品名：D-果糖/D-葡萄糖檢測試劑盒

品名：D-Fructose plus D-glucose assay kit

規(guī)格：110 次檢測 (手工) / 1100 (微孔板) / 1100 (自動分析儀)

應用：D-果糖/D-葡萄糖檢測試劑盒是一種酶法-紫外檢測方法，用于測量和檢測植物和食品中D-果糖,和/或D-葡萄糖含量。輔酶因子更強的穩(wěn)定性，溶解后的輔酶因子在4°C下可保存超過1年。適合手工、微孔板和自動分析儀分析。

原理：

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

檢測方法：分光光度法 @340 nm

反應時間：~13min       子起生物:

檢測限：0.66 mg/L

應用案例：

葡萄酒、啤酒、果汁、軟飲料、牛奶、果醬、蜂蜜、食療食品、面包、焙烘食品、糖果、甜點、糕點、冰淇淋、水果蔬菜、調(diào)味品、煙草、化妝品、藥品、造紙和其他原料(如生物培養(yǎng)、樣品等)

方法識別：

基于上述原理的檢測方法已經(jīng)被AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK和IOCCC等組織接受。

試劑盒組成：

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

Bottle 1: 緩沖溶液(15 mL, pH 7.5) 添加疊氮鈉(0.02%)作為防腐劑

  4°C下穩(wěn)定超過 2年

Bottle 2: NADP+, ATP和PVP。 子起生物:

  -20°C穩(wěn)定超過 5年

Bottle 3: 己糖激酶和葡萄糖-6-磷酸脫氫酶懸浮液, 2.25mL.

  4°C下穩(wěn)定超過 2年

Bottle 4: 磷酸葡萄糖異構酶懸浮液(2.25 mL)。

  4°C下穩(wěn)定超過 2年

Bottle 5: D-葡萄糖和D-果糖標準溶液(5 mL, 每種糖濃度為0.2 mg/mL)。

  4°C下穩(wěn)定超過 2年

優(yōu)點：

包含PVP可預防單寧抑制

經(jīng)過法國Suze la Rousse葡萄酒大學驗證

價格非常具有競爭力

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

所有試劑制備后可以穩(wěn)定保存超過2年（針對手工分析應用）

在25°C或37°C下都能快速檢測 提供Mega-Calc? 軟件工具用于一站式原始數(shù)據(jù)處理

包含標準溶液

輔酶因子更強的穩(wěn)定性       子起生物:

適合手工、微孔板和自動分析儀分析。

參考文獻：

Grape and wine analysis: Oenologists to exploit advanced test kits. Charnock, S. C. & McCleary, B. V. (2005). Revue des Enology, 117, 1-5.

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

Megazyme “advanced” wine test kits general characteristics and validation. Charnock, S. J., McCleary, B. V., Daverede, C. & Gallant, P. (2006). Reveue des Oenologues, 120, 1-5.

Lactose fermentation by Kombucha – a process to obtain new milk–based beverages. Ili?i?, M., Kanuri?, K., Milanovi?, S., Lon?ar, E., Djuri?, M. & Malba?a, R. (2012). Romanian Biotechnological Letters, 17(1), 7013-7021.

Sourdough-leavened bread improves postprandial glucose and insulin plasma levels in subjects with impaired glucose tolerance. Maioli, M., Pes, G. M., Sanna, M., Cherchi, S., Dettori, M., Manca, E. & Farris, G. A. (2008). Acta Diabetologica, 45(2), 91-96.

Endocrine and metabolic effects of consuming fructose- and glucose-sweetened beverages with meals in obese men and women: Influence of insulin resistance on plasma triglyceride responses. Teff, K. L., Grudziak, J., Townsend, R. R., Dunn, T. N., Grant, R. W., Adams, S. H., Keim, N. L., Cummings, B. P., Stanhope, K. L. & Havel, P. J. (2009). Journal of Clinical Endocrinology & Metabolism, 94(5), 1562-1569.

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

Metabolic and endocrine profiles in response to systemic infusion of fructose and glucose in rhesus macaques. Adams, S. H., Stanhope, K. L., Grant, R. W., Cummings, B. P. & Havel, P. J. (2008). Endocrinology, 149(6), 3002-3008.

β-Fructofuranosidase and sucrose phosphorylase of rumen bacterium Pseudobutyrivibrio ruminis strain 3. Kasperowicz, A., Stan-Glasek, K., Guczynska, W., Pristas, P., Javorsky, P., Vandzurova, A. & Michalowski, T. (2012). World Journal of Microbiology and Biotechnology, 28(3), 1271-1279.     子起生物:

Comparative effects of fructose and glucose on lipogenic gene expression and intermediary metabolism in HepG2 liver cells. Hirahatake, K. M., Meissen, J. K., Fiehn, O. & Adams, S. H. (2011). PloS one, 6(11), e26583.

Measurement of glucose and fructose in clinical samples using gas chromatography/mass spectrometry. Wahjudi, P. N., Patterson, M. E., Lim, S., Yee, J. K., Mao, C. S. & Lee, W. N. P. (2010). Clinical Biochemistry, 43(1-2), 198-207.

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit 

Physiological studies of Leuconostoc mesenteroides strain NRRL B-1149 during c*tion on glucose and fructose media. Bivolarski, V., Vasileva, T., Shukla, R., Goyal, A. & Iliev, I. (2012). Journal of BioScience & Biotechnology, 1(3), 235-240.

Aldose reductase is implicated in high glucose‐induced oxidative stress in mouse embryonic neural stem cells. Fu, J., Tay, S. S. W., Ling, E. A. & Dheen, S. T. (2007). Journal of Neurochemistry, 103(4), 1654-1665.

Immobilised yeast grape must deacidification in a recycle fixed bed reactor. Portugal, I., Ribeiro, S. C., Xavier, A. M. R. B., Centeno, F. & Strehaiano, P. (2011). International Journal of Food Science & Technology, 46(2), 284-289.

The Elemental Composition of Synechocystis sp. PCC6803 under Nutrient Limiting Conditions.Blom, M. (2014). University of Amsterdam, Bachelor Thesis.

K-FRUCL D-果糖/D-葡萄糖檢測試劑盒 , D-Fructose plus D-glucose assay kit