現(xiàn)貨產(chǎn)品人Ⅰ型前膠原N端前肽(PⅠNP)ELISA檢測試劑盒全國:歡迎選購!,,到我司741653262,公司提供*進(jìn)口,國產(chǎn)elisa試劑盒,均為高性價(jià)比試劑盒高品質(zhì)elisa試劑盒,齊一生物科技有限公司提供的試劑盒受到了廣大科研單位的*肯定和認(rèn)同.*保證,價(jià)格公道傾力為國內(nèi)外科研院校實(shí)驗(yàn)室提供的產(chǎn)品
現(xiàn)貨產(chǎn)品人Ⅰ型前膠原N端前肽(PⅠNP)ELISA檢測試劑盒類別:酶聯(lián)免疫分析檢測試劑盒
規(guī)格:96T/48T
包裝:盒
檢測原理:采用雙抗夾心法
保質(zhì)期:六個(gè)月
產(chǎn)品保存條件:2-8°C
應(yīng)用范圍:僅供科研研究實(shí)驗(yàn)使用
樣品形式:血清/血漿/組織勻漿/細(xì)胞培養(yǎng)上清//其它生物液體(具體情況請咨詢)
標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測分泌性的成份時(shí),用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測,其余冷凍備用。
Consumable 79000 Oligotex Suspension (0.5) 0.5 ml for mRNA isolation from up to 8 mg of total RNA
Consumable 203205 HotStarTaq DNA Polymerase (1000) "4 x 250 units HotStarTaq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
FOR MORE INFO ABOUT THIS PRODUCT PLEASE SEE HOTSTARTAQ DNA POLYMERASE 250 UNITS (203203)"
Consumable 210210 QIAGEN OneStep RT-PCR Kit (25) For 25 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, 5x QIAGEN OneStep RT-PCR Buffer,* dNTP Mix,** 5x Q-Solution, RNase-free water
Consumable 301307 SuperFect Transfection Reagent(4x1.2 ml) For 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
Consumable 28206 MinElute Reaction Cleanup Kit (250) 250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Instrument Part 9015637 AC fuse, 230 V, 4A, M48
Instrument Part 9016560 BR MDx DSP Maint.pro Plasticware Kit, V1
Instrument Part 9239360 Z-Axis Coupling, EZ1
Consumable 132605 NeXtal Stock Sodium ac. H2O (50) 1.6M Sodium acetate trihydrate - 50mL (4 x 12,5 ml tube)
Instrument Part 9019581 Pulley, Idler Y-axis, Cxx
Consumable 9019070 PyroMark Thermoplate Low, PyroQ96ID Holder for placement of PyroMark Q96 Plate Low on heat block during annealing step prior to PyroMark Q96 ID analysis.
Instrument Part 9019656 Hose w. quick lock socket, PyroQ96VW
Consumable 972022 PyroMark Q96 CpG MLH1 (96) For quantification of methylation level in MLH1 promoter
Consumable 979103 PyroMark Q96 HS Nucleotide Tip (8) Re-usable tips for dispensing nucleotides (NDTs) for use in PyroMark Q96 MD. Each package contains 8 tips.
Instrument Part 9017763 User Manual EZ1 DSP
Instrument Part 9240635 Filter, EMI,Modular, RJ45, AP196/98
Instrument Part 9240766 32 OUT Module, D2-32TD1, AP96
Instrument Part 9018125 Magnet plate, extractor, Qsym
Instrument Part 9018133 Sensor, rodcover, extractor Qsym
Instrument Part 9018134 Sensor, cartridge, extractor, Qsym
Instrument Part 9018146 Motor, z, heated shaker, Qsym
Instrument Part 9240955 Sensor, z, handler, Qsym
Consumable 765512 PAXgene Tissue STABILIZER Conc. (150 ml)
Instrument Part 9022818 EZ1 Advanced XL Flip Cap Tube Rack
Consumable 9243773 QIAsymphony AS Custom Protocol Basic2
Instrument Part 9238697 Eprom 4K15, Zent2, imbalance sensor 9238697 is a microchip that plugs into the microcontroller board which controls the imbalance sensor.
Instrument Part 9238714 Front panel, Zent1
Instrument Part 9238800 Bucket for up to 3 microplates Sigma 13220
Instrument Part 9238819 Rect. carrier for 5 glass tubes 25ml
9240102 LiquiChip, Re-Installation The Re-Installation, LiquiChip facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware and software check of instrument and provides user with operator refesher overview. Re-installation includes labor and travel costs. Re-installation performance process is documented on a GMP/GLP-pliant Field Service document.
Instrument Part 9239073 Syringe 1,0 ml, Kloehn
Consumable 301807 HiPerFect HTS Reagent (6 x 1 ml)
Consumable 285215 cador B. hyodysenteriae PCR Reagent (96)
Consumable 59760 EpiTect Fast 96 LyseAll Bisulfite Kit
Instrument Part 9014835 AC-Box, 400W, BR8, RD v2.0
Consumable 60704 QIAamp DSP Virus Kit (50),CE
Consumable 940054 BioSprint 96 DNA Blood Kit (48)
Instrument Part 9238645 Brake resistor 112 Ohm / 100 W
Instrument Part 9238659 Cooling pressor T2168GK, 60Hz
Instrument Part 9238662 Lid with window
Instrument Part 9238664 Hinge
Consumable 132983 NeXtal Stock Kit Buffer 48 x 10 ml tubes containing solutions with a wide range of final pH (2,2 to 11,6)
Consumable 204141 QuantiTect SYBR Green PCR Kit (40) "Trial kit size for 40 x 50 ul reactions: 1.7 ml QuantiTect SYBR Green PCR Master Mix, 2.0 ml RNase-free water
For quantitative, real-time PCR and two-step RT-PCR using SYBR Green detection."
Instrument Part 9015471 Sensor, photo, PM-U24
9016234 BGR Zentrieradapter
Accessory 19581 96-Well Microplates (24) 96-well microplates with round-bottom wells plus lids, 24 per case, for use with QIAvac manifolds and the BioRobot 9600
Consumable 28606 MinElute Gel Extraction Kit (250) 250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Consumable 34450 RGS.His HRP Conjugate Kit 125 ul RGS-His HRP Conjugate, 5 g Blocking Reagent, 50 ml Blocking Reagent Buffer (10x concentrate)
Consumable 35023 Ni-NTA HisSorb Strips (24) 2 racks of 12 x Ni-NTA-coated 8-well strips in 96-well format