齊一生物鐵蛋白(FE)ELISA檢測試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個試劑盒都經(jīng)過嚴格的質(zhì)檢控制,包括:板內(nèi)、板間實驗精度,靈敏度,回收率,穩(wěn)定性等指標的測定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測體系簡單而直接,可根據(jù)450 nm波長的讀值迅速計算定量結(jié)果。試劑盒中提供的檢測抗體能夠識別全長靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測結(jié)果更穩(wěn)定可靠
齊一生物鐵蛋白(FE)ELISA檢測試劑盒標本要求:
1.標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
注意事項:
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標準曲線,做復(fù)孔。如標本中待測物質(zhì)含量過高(樣本OD值大于標準品孔*孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準。
計算:
以標準物的濃度為橫坐標,OD值為縱坐標,
在坐標紙上繪出標準曲線,根據(jù)樣品的OD
值由標準曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標準物的濃度與OD值計算出標
準曲線的直線回歸方程式,將樣品的OD值
代入方程式,計算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實際濃度。
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)與批見應(yīng)分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個月 齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,購買我公司產(chǎn)品的新老客戶,可享免費代測實驗服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本。全程提供。如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議。
Instrument Part 9240764 DC Output Module 8NPT, 3AMP, AP96
Instrument Part 9240668 Nut, Flangeless,1/4-28, Blue, AP
Instrument Part 9240768 Signal Conditioner, Centrifuge, AP96
Instrument Part 9018132 Splash guard, extractor, Qsym
Instrument Part 9018135 Sensor, xz, et/hs, Qsym
Instrument Part 9019901 Lead Screw - X, PyroQ96ID
Instrument Part 9019014 Heating block, EZ1xl
Instrument Part 9019312 Solenoid, door lock, EZ1xl
Instrument Part 9019023 Worktable, front bar, EZ1xl
Instrument Part 9019926 PCB, Microboard, Cxx
Instrument Part 9019927 Cover, tip ejector (UV), Cx8
Consumable 979017 MOTT 16S Primer 2 For Sequencing
950163 Final Wash Reagent (DXF) (350 ml)
Instrument Part 9021624 Kit, 8 ch pip. plunger upgrade, Cx8
Replacement Component 1031240 REPLI-g Midi-Reacti.Buffer(730μl/2),KG
Consumable 35061 Ni-NTA HisSorb Plates (5) 5 Ni-NTA-coated, transparent 96-well plates
Consumable 51504 QIAamp DNA Stool Mini Kit (50) For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, InhibitEX tablets, Buffers, Collection Tubes (2 ml)
Consumable 205110 Omniscript RT Kit (10) For 10 reverse-transcription reactions: 40 units Omniscript Reverse Transcriptase, 10x Buffer RT, dNTP Mix,* RNase-free water
Consumable 963141 QIAquick 96 PCR BioRobot Kit (4) For purification of 4 x 96 PCR products: 4 QIAquick 96 Plates, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads
Replacement Component 1011132 RDD Buffer Set for RNeasy 96 "as per TS DE
35 ml Buffer RDD and 7 ml RNase-free water"
Instrument Part 9239151 Screw, pod fixation (to support), TWII
Instrument Part 9239362 Inlet-micro-fuse T 6.3A, 250V, EZ1
Consumable 132600 NeXtal Stock Potassium sodium tar. (50) 1.5M Potassium sodium tartrate tetrahydrate - 50mL (4 x 12,5 ml tube)
Consumable 132738 NeXtal Stock Dioxane (200) 100% v/v Dioxane - 200mL (20 x 10 ml tube)
Instrument Part 9019579 LED, power assembly, C1x
Instrument Part 9019625 PCB, level sensor rev8, Cx2
Consumable 9019088 PyroMark IdentiFire SW 1.0 (5) "Software for sequence-based species identification by paring and matching Pyrosequencing data to a local database.
Five additional software licenses for PyroMark IdentiFire SW 1.0"
Consumable 970032 PyroMark Q24 CpG MGMT (4 x 24) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.
Consumable 979001 PyroMark Q96 Plate (100) "For sample analysis of DNA template prepared with magnetic beads for ID.
Magnetic bead technology instead of vacuum prep tool is used here"
Consumable 979203 PyroMark Control Oligo For installation check of PyroMark instrument.
Instrument Part 9019455 Upgrade kit GD100, incl. Perm/Remove, HS
Consumable 950901 Capture Plate, standard yield (5)
Instrument Part 990554 Reagent Trough (with Lid), 70 mL (10) ccc
Instrument Part 9240765 32 IN Module, D2-32ND3, AP96
Instrument Part 9240767 8 OUT Relay, F2-08TRS, AP96
Instrument Part 9240770 Assy, Capper Shuttle, AP96
Instrument Part 9017435 Sample tracking, Qsym
Instrument Part 9018149 Piercing, consumable drawer, Qsym
9018156 Tip slide, waste drawer, Qsym
Instrument Part 9018157 Holder, tip bag, waste drawer, Qsym
Instrument Part 9240950 PCB, xyz, portal, Qsym
Instrument Part 9240983 Cooling device, eluate drawer, Qsym
Instrument Part 9240984 PCB, p&c, cooling device, Qsym
929707 QX Cartridge Stand Cover (1)
Consumable 218161 miScript II RT Kit (50)
Consumable 873001 IDH 1/2 RGQ PCR kit (20)
Instrument Part 9238682 #Service manual Zent3, english
Instrument Part 9238696 LED display HD1133
Instrument Part 9238707 Temperature sensor
Instrument Part 9238785 Swing-out rotor 72 x 15 ml plete
Instrument Part 9238809 Round carrier for 4 Falcon tubes 50ml
Instrument Part 9238820 Rect. carrier for 2 tubes 50 ml
Instrument Part 9235700 Power Cable 24V, RCS1
Consumable 206145 QIAGEN Multiplex PCR Kit (1000) "contains
1 x Multiplex PCR MasterMix 2x, (1 x 25 ml)
(Mastermix contains HotStarTaq? DNA Polymerase, Multiplex PCR Buffer*, dNTP Mix)
1 x Q-Solution (10ml)
1 x RNase-free water (20 ml)
Handbook"
Instrument Part 9017186 Plunger set (6), Biorobot EZ1
9240103 BR MDX DSP, Re-Installation The Re-Installation, BR MDx DSP facilitates quick implementation of system back into lab routine by minimizing instrument set-up phase. It covers hardware and software check of instrument and provides user with operator refesher overview. Re-installation includes labor and travel costs. Re-installation performance process is documented on a GMP/GLP-pliant Field Service document.
Instrument Part 9016493 BR MDx DSP Service, PRO
Instrument Part 9239071 Valve 3-way, Kloehn
MDx 764133 PAXgene Bone Marrow RNA Kit (30) "For storage and transport of bone marrow samples and stabilization and purification of intracellular RNA
Integrated system for storage, stabilization, and purification
Immediate stabilization of intracellular RNA at collection
Rapid purification from heterogeneous bone marrow samples
Safe and easy transportation"
Consumable 337831 qBiomarker SYBR Fluor Mastermix (8)
Consumable 387116 Investigator Quantiplex HYres Kit (200)
9021147 Bottle, Reagent (with cap assy), DCU
Instrument Part 9238246 Elution Tube Rack, 1.5 ml
Instrument Part 9015076 Probe, stainless steel, 0.9mm, 138 mm
Consumable 970452 PyroMark Q24 SNP KRAS v2.0 (4 x 24) For detection of mutations in codon 12, codon 13 and codon 61.optimized for FFPE material
Consumable 972032 PyroMark Q96 CpG MGMT (96) For quantification of methylation level in region 17-39 of exon 1 of the MGMT gene.
Consumable 979006 PyroMark Binding Buffer (200 ml) For immobilization of biotinylated DNA to streptavidin coated Sepharose beads.
Consumable 979104 PyroMark Q96 HS Capillary Tip (8) "Re-usable capillary tips for dispensing nucleotides (CDTs) for use in PyroMark Q96 MD. Each package contains 8 tips
CDT = capillary dispensing tips"
Instrument Part 9019460 Heater assy incl. element & sensor, HS
Instrument Part 981400 Rotor-Disc OTV Kit (1) "Kit for optical temperature verification of Rotor-Gene systems (includes a Rotor-Disc preloaded with thermochromatic liquid crystals, fluorescent inserts, CD with calibration files)
REQUIRES ROTOR-DISC 72 ROTOR AND LOCKING RING OR ROTOR-DISC 72 STARTER KIT"
Instrument Part 9017841 Tool, rotor fixation, calibration, QIAC
Instrument Part 9022664 Channeling Block Holder, QXT
Consumable 12381 EndoFree Plasmid Mega Kit (5) 5 QIAGEN-tip 2500, Reagents, 5 QIAfilter Mega-Giga Cartridges, Endotoxin-free Buffers
Accessory 19560 Collection Microtubes (racked) Nonsterile polypropylene tubes (1.2 ml), 960 in racks of 96
Consumable 30250 Ni-NTA Agarose (500 ml) 500 ml nickel-charged resin (max. pressure: 2.8 psi)
Consumable 13343 Blood & Cell Culture DNA Midi Kit 25 QIAGEN Genomic-tip 100/G, QIAGEN Protease, Buffers
Consumable 74106 RNeasy Mini Kit (250) 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable 19075 Buffer AL 216 ml for 1000 preps
Accessory 19157 QIAGEN Protease (30 AU) 4 x 7.5 Anson units per vial (lyophilized)
Accessory 19583 24-Well Blocks RB (24) 24-well blocks with 10 ml round-bottom wells, 24 per case
Consumable 28604 MinElute Gel Extraction Kit (50) 50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Consumable 34530 Ni-NTA HRP Conjugate Horseradish-peroxidase-conjugated Ni-NTA (lyophilized, for 500 ml working solution)
Accessory 34964 Polypropylene Columns (5-ml) 50/pack, 5 ml capacity
Instrument Part 9018095 QIAcube Transport Box
Instrument Part 9240568 Centrifuge Locator Cylinder, AP96
Instrument Part 9240649 Internal BCR, Keyence, BL-601, AP196/98
Instrument Part 9240697 Adaptor, Waste Drain, AP
Instrument Part 9240680 Spill Tray, Shaker,Modified, AP
Instrument Part 9018163 Electronic box, Qsym
Instrument Part 9018139 Tray, extractor, Qsym
Instrument Part 9018153 Waste drawer, Qsym
Instrument Part 9240968 Barcode camera, sample tracking, Qsym
Instrument Part 9240976 PCB, display, sample tracking, Qsym
Consumable 19419 QIAvac Connecting System System to connect vacuum manifold with vacuum pump: includes Tray, Waste Bottles, Tubings, Couplings, Valve, Gauge, 24 VacValves
Instrument Part 9238605 Centrifuge Motor, plete
Instrument Part 9238631 Keypad, foil
Instrument Part 9238647 Mechanical lock with optic, left