說明書下載QFDN.rar 分類名稱 : 代謝產(chǎn)物分析 產(chǎn)品介紹 : 測(cè)定方法 熒光法(340nm/450nm)定量測(cè)定DNA的濃度。 Description DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling.Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. our QuantiFluoTM DNA assay kit is designed to accuray measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at 450nm (lexc = 350nm), is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples. Key features Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 – 2,000 ng/mL) calf thymus DNA in 96-well plate assay.Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay. Applications: Direct Assays: plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices. Storage conditions. The kit is shipped at room temperature. Store the DNA standard at -20°C and the Reagent at 2-8°C. Shelf life of at least 12 months (see expiry dates on labels). 其他 :編號(hào):QFDN-048,QFDN-250 測(cè)試:48T,250T |
北京鴻躍創(chuàng)新科技有限公司是一家經(jīng)營(yíng)科研產(chǎn)品代理、銷售生物試劑、生物耗材、生物儀器等于一身的生物技術(shù)企業(yè),其產(chǎn)品覆蓋了生命領(lǐng)域從分子生物學(xué)研究、蛋白表達(dá)、細(xì)胞培養(yǎng)到層析純化,從抗原抗體、成套試劑盒到生物培養(yǎng)及其生物活性研究等相關(guān)試劑幾近所有過程.
在生物公司林立的今天,鴻躍公司始終堅(jiān)持以“*,信譽(yù)*”的服務(wù)宗旨.憑借其對(duì)市場(chǎng)的充分了解,對(duì)產(chǎn)品深入細(xì)致的分析,以其雄厚的資本,快速的流通方式,隨時(shí)為客戶提供*、技術(shù)含量高、操作方便快捷的實(shí)驗(yàn)急需產(chǎn)品.
鴻躍公司在為廣大科學(xué)工作者們提供產(chǎn)品的同時(shí)也注重公司員工自身的素質(zhì)培養(yǎng).不但要求員工有扎實(shí)的專業(yè)基礎(chǔ),還要有較高的文化素質(zhì)和道德修養(yǎng).強(qiáng)調(diào)員工與客戶建立良好的合作關(guān)系,公司內(nèi)部的團(tuán)隊(duì)合作精神.公司規(guī)模不求較大,但求Z佳,價(jià)格不求Z低,但求合理.
總之,鴻躍公司將以“更加執(zhí)著地追求客戶的滿意度”為宗旨,全心全意地為客戶做好每一件事.同是,對(duì)一直以來關(guān)心鴻躍公司的朋友們深表感謝!讓我們與生命科學(xué)攜手共步美好的明天!
經(jīng)營(yíng)范圍
現(xiàn)貨提供兩千多種包括Sigma、Aldrich、Fluka、 Amresco、Merck、Roche、Serva、Promega、Pharmacia、 Cambrex(BioWhittaker)、Gibco、HyClone、BMA、Qbiogene、Oxid、BioLabs、Acros、Difco、等國(guó)外*公司的產(chǎn)品,并承擔(dān)以上公司專項(xiàng)訂貨業(yè)務(wù)。
二.分子生物學(xué)成套試劑盒
現(xiàn)貨提供美國(guó)Omega、Qbiogene(Bio101)、Promega核酸分離和純化試劑盒,自產(chǎn)試劑盒。期貨提供德國(guó)Qiagen、MoBiTec、美國(guó)Ambion試劑盒。
三.免疫學(xué)試劑及檢測(cè)試劑盒
*現(xiàn)貨提供三千多種Sigma、R&D、Zymail、UsBio、Santa Curz、Maixin-Bio進(jìn)口分裝抗體及免疫組化配套試劑。Biosoure、R&D 、Diaclone等公司進(jìn)口分裝Elisa Kit、Eli-spot Kit。Biotium分子探針。各種國(guó)產(chǎn)及進(jìn)口分裝二抗,國(guó)產(chǎn)檢測(cè)試劑盒及細(xì)胞因子等。
期貨提供產(chǎn)品包括Santa Cruz、Calbiochem等公司的大包裝抗體和 Maixin-Bio、Affiniti、Neomarkers、Genx BioSciense、Zyman、UsBio等公司的抗體;KPL、Santa Cruz等公司的二抗;Maixin-Bio等公司的免疫組化配套試劑;Diaclone、 IBL、 MBL、Endogen、DSL、Biosource等公司的Elisa Kit;Diaclone公司的Eli-spot Kit;Biotium、MoiecularProbe公司探針;Peprotech,、R&D、Sigma、Calbiochem等公司的細(xì)胞因子。
四.細(xì)胞生物學(xué)產(chǎn)品
現(xiàn)貨部分提供美國(guó)Gibco、HyClone、Oxoid公司的培養(yǎng)基、血清、等培養(yǎng)試劑。并承擔(dān)以上公司專項(xiàng)訂貨業(yè)務(wù)。
五.試驗(yàn)耗材
現(xiàn)貨提供德國(guó)Sartorius,英國(guó)Whatman等過濾及超濾產(chǎn)品?,F(xiàn)貨提供美國(guó)Corning Costar、B.D、日本IWAKI、比利時(shí)Orange、丹麥NUCK、德國(guó)Greiner等細(xì)胞培養(yǎng)耗材?,F(xiàn)貨提供美國(guó)Axygen、Qsp、德國(guó)Brand、SCHOTT、芬蘭Biohit、美國(guó)Fisher等常規(guī)耗材。現(xiàn)貨提供各種國(guó)產(chǎn)耗材。
六.實(shí)驗(yàn)儀器及設(shè)備
現(xiàn)貨提供進(jìn)口原裝芬蘭百得(Biohit),雷勃,大龍移液產(chǎn)品;德國(guó)Brand、Eppendorf;法國(guó)Gilson;日本Nichiryo等國(guó)移液設(shè)備。提供各種進(jìn)口及國(guó)產(chǎn)實(shí)驗(yàn)儀器及設(shè)備。
- 面議在線詢價(jià)
產(chǎn)品名稱參考價(jià)