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德國(guó)Nova剛地弓形蟲(chóng)第二代檢測(cè)試劑盒

剛地弓形蟲(chóng)是一種無(wú)處不在的原蟲(chóng),世界范圍內(nèi)的分布。這個(gè)專性細(xì)胞內(nèi)寄生蟲(chóng)可以感染人類,以及幾乎所有的溫血?jiǎng)游?/span>,包括哺乳動(dòng)物和鳥(niǎo)類。弓形蟲(chóng)的生命周期是兼性heteroxenous。中間宿主可能是所有溫血?jiǎng)游锇ù蟛糠稚?/span>,和人類。明確的主機(jī)是貓科家族的成員,例如家貓。

有三個(gè)感染弓形蟲(chóng)的生命周期階段,即個(gè)速、bradyzoites(在組織囊腫),和子孢子(形成孢子卵囊)。所有三個(gè)階段都是傳染性中間和zui終的主機(jī)。

弓形蟲(chóng)感染可能是收購(gòu)主要是通過(guò)以下路線之一:口服攝入的傳染性卵囊的環(huán)境,通過(guò)口服攝入的組織中包含的囊腫中間宿主的生的或未煮熟的肉類,或經(jīng)胎盤(pán)的個(gè)的傳播。

雖然在人類感染弓形蟲(chóng)很常見(jiàn),臨床疾病在很大程度上局限于高危人群。大多數(shù)感染弓形蟲(chóng)免疫活性的人類是無(wú)癥狀的。

然而,如果*次懷孕期間感染,弓形蟲(chóng)可能會(huì)傳染給胎兒。先天性弓形體病可能導(dǎo)致流can、新生兒死亡或胎兒異常對(duì)胎兒有害的后果。如果不及時(shí)治療,胎兒的宮內(nèi)感染風(fēng)險(xiǎn)增加在懷孕期間。

疾病	癥狀	感染的機(jī)制
后天獲得的弓形體病	免疫活性的患者: 通常無(wú)癥狀或非常溫和非特異性的癥狀包括頸部淋巴結(jié)病和其他感染廣義的癥狀(發(fā)熱、不適、盜汗,肌痛,喉嚨痛和斑丘疹的皮疹)	攝入肉或內(nèi)臟組織囊腫感染
重新激活弓形體病	免疫功能低下的患者: 腦炎、心肌炎、肺炎	卵囊在貓科動(dòng)物糞便污染的食物或水
先天性弓形體病	先天感染新生兒: 脈絡(luò)膜視網(wǎng)膜炎、失明、癲癇、精神發(fā)育遲滯、貧血、黃疸、腦炎、肺炎等 *恢復(fù)是非常罕見(jiàn)的	先天性傳播:如果懷孕期間發(fā)生感染的急性期,這種寄生蟲(chóng)可以穿過(guò)胎盤(pán)和胎兒感染

感染的診斷則需要通過(guò):

血清學(xué):確定特定抗體的ELISA、間接熒光抗體試驗(yàn)(IFAT)免疫吸附凝集試驗(yàn)(ISAGA)
寄生蟲(chóng)檢測(cè):聚合酶鏈反應(yīng)

NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g ELISA:

的NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g ELISA定量測(cè)定的目的是IgG-class剛地弓形蟲(chóng)抗體在人類血清或血漿(檸檬酸)。

NovaLisa®剛地弓形蟲(chóng)IgMµ-capture ELISA:

的NovaLisa®剛地弓形蟲(chóng)IgMµ-capture ELISA用于定性測(cè)定IgM-class剛地弓形蟲(chóng)抗體在人類血清或血漿(檸檬酸)。

抗原:

剛地弓形蟲(chóng)溶解產(chǎn)物(免疫球蛋白),剛地弓形蟲(chóng)嵌合抗原(IgM)

具體的性能特征:

	Intraassay			Interasay			靈敏度%	特異性%
	n	的意思是	CV %	n	的意思是	CV %
免疫球蛋白	6	0.978	5.7	4	0.752	7.1	96.6	98.2
	6	1.647	5.3	4	1.26	10.1
	6	2.496	6.2	4	1.98	6.3
IgM µ-capture	24	1.563	2.9	12	7.08	6.5	95.8	> 98
IgM µ-capture	24	0.304	1.5	12	16.68	4.5	95.8	> 98

NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g貪欲測(cè)試*:

貪欲的決心是一種診斷方法,用于區(qū)分zui近(急性)和更遙遠(yuǎn)的(過(guò)去的)病人血清感染剛地弓形蟲(chóng)。貪欲的約束力是抗體與相應(yīng)抗原(血清標(biāo)本)。

免疫球蛋白的測(cè)定貪欲依賴的進(jìn)步增加親和力的抗體靶抗原的過(guò)程中天然免疫后感染。測(cè)量抗體綁定的強(qiáng)度是一種證實(shí)或排除近期感染。Low-avidity抗體從zui近收購(gòu)了弓形蟲(chóng)感染剛地可以從high-avidity分化抗體,這是一個(gè)指示性的感染。

換句話說(shuō),低的免疫球蛋白抗體在感染的早期階段可以從高分化的抗體與過(guò)去的感染有關(guān)。

IgG抗體活性的測(cè)定是一個(gè)額外的分析經(jīng)典的血清學(xué)關(guān)于剛地弓形蟲(chóng)感染的狀態(tài)。

的NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g貪欲測(cè)試是一種額外的試劑盒與NovaLisa結(jié)合使用®剛地弓形蟲(chóng)免疫球蛋白g ELISA。

性能特點(diǎn):

的NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g貪欲測(cè)試被用于評(píng)估弓形體病急性和過(guò)去感染樣品?？倲?shù)量86定義病人樣本測(cè)試。這些樣本提供的醫(yī)學(xué)微生物學(xué)研究所免疫學(xué)和寄生蟲(chóng)學(xué),波恩大學(xué)。

		定義樣本
		急感染	過(guò)去的感染	總	協(xié)議 (%)
NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g貪欲測(cè)試	急感染	36	1	37	94.7
	過(guò)去的感染	2	47	49	97.9
	總	38	48	86年	96.5

訂單信息:

ELISA	的數(shù)量決定	產(chǎn)品編號(hào)
弓形蟲(chóng)免疫球蛋白	96年	TOXG0460
弓形蟲(chóng)IgMµ-capture	96年	TOXM0460
弓形蟲(chóng)免疫球蛋白g貪欲測(cè)試*	96年	TOXGA460

* NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g貪欲測(cè)試是一種額外的試劑,用于結(jié)合NovaLisa®弓形蟲(chóng)剛免疫球蛋白ELISA。

RiliBAK

與Liquickeck®火炬+控制(Bio-Rad)外部控制可用NovaLisa®剛地弓形蟲(chóng)免疫球蛋白g

【公司名稱】廣州健侖生物科技有限公司
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Toxoplasma gondii 2nd Generation

Toxoplasma gondii is a ubiquitous protozoon that has a worldwide distribution. This obligate intracellular parasite can infect humans as well as virtually all warm-blooded animals, including mammals and birds. The life cycle of T. gondii is facultatively heteroxenous. Intermediate hosts are probably all warm-blooded animals including most livestock, and humans. Definitive hosts are members of the family Felidae, for example domestic cats.

There are three infectious stages in the life cycle of T. gondii, i.e. tachyzoites, bradyzoites (in tissue cysts), and sporozoites (in sporulated oocysts). All three stages are infectious for both intermediate and definitive hosts.

T. gondii infections may be acquired mainly via one of the following routes: by oral ingestion of infectious oocysts from the environment, by oral ingestion of tissue cysts contained in raw or undercooked meat of intermediate hosts, or by transplacental transmission of tachyzoites.

While infection with T. gondii in humans is very common, clinical disease is largely confined to risk groups. Most T. gondii infections in immunocompetent humans are asymptomatic.

However, if first contracted during pregnancy, T. gondii may be transmitted to the foetus. Congenital toxoplasmosis may cause abortion, neonatal death, or foetal abnormalities with detrimental consequences for the foetus. If not treated, the risk of intrauterine infection of the foetus increases during pregnancy.

Disease	Symptoms	Mechanism of Infection
Postnatally acquired toxoplasmosis	Immunocompetent patients: Usually asymptomatic or very mild Nonspecific symptoms including cervical lymphadenopathy and other symptoms of generalized infection (fever, malaise, night sweats, myalgia, sore throat and maculopapular rash)	Ingestion of tissue cysts in infected meat or viscera
Reactivated toxoplasmosis	Immunocompromised patients: encephalitis, myocarditis and pneumonitis	Oocysts in food or water contaminated with feline faeces
Congenital toxoplasmosis	Congenitally infected newborns: chorioretinitis, blindness, epilepsy, mental retardation, anemia, jaundice, encephalitis, pneumonitis and others Complete recovery is very rare	Congenital transmission: if the acute phase of the infection occurs during pregnancy, the parasite can cross the placenta and infect the fetus

Infections may be diagnosed by:

Serology: Determination of specific antibodies by ELISA, indirect fluorescence antibody test (IFAT) immunosorbent agglutination assay (ISAGA)
Parasite detection: PCR

NovaLisa® Toxoplasma gondii IgG ELISA:

The NovaLisa^® Toxoplasma gondii IgG ELISA is intended for the quantitative determination of IgG-class antibodies against Toxoplasma gondii in human serum or plasma (citrate).

NovaLisa® Toxoplasma gondii IgM µ-capture ELISA:

The NovaLisa® Toxoplasma gondii IgM µ-capture ELISA is intended for the qualitative determination of IgM-class antibodies against Toxoplasma gondii in human serum or plasma (citrate).

Antigens:

Toxoplasma gondii lysate (IgG) and Toxoplasma gondii chimeric antigens (IgM)

Specific performance characteristics:

	Intraassay			Interasay			Sensitivity%	Specificity%
	n	Mean	CV%	n	Mean	CV%
IgG	6	0.978	5.7	4	0.752	7.1	96.6	98.2
	6	1.647	5.3	4	1.26	10.1
	6	2.496	6.2	4	1.98	6.3
IgM µ-capture	24	1.563	2.9	12	7.08	6.5	95.8	>98
IgM µ-capture	24	0.304	1.5	12	16.68	4.5	95.8	>98

NovaLisa® Toxoplasma gondii IgG Avidity Test*:

The avidity determination is a diagnostic method which is used to differentiate a recent (acute) and a more distant (past) infection with Toxoplasma gondii in patient sera. Avidity is the binding force of the antibody (serum specimen) with the corresponding antigen.

The determination of IgG avidity relies on the progressive increase of the affinity of an antibody for its target antigen during the course of natural immunity following an infections. Measuring the strength of the antibody binding is a way to confirm or rule out a recent infection. Low-avidity antibodies from a recent acquired Toxoplama gondii infection can be differentiated from high-avidity antibodies, which is an indicative of past infection.

In other words, low avid IgG antibodies in the early stage of infection can be differentiated from high avid antibodies associated with a past infection.

The determination of IgG antibody avidity is an additional analysis to the classic serology in regards to the status of a Toxoplasma gondii infection.

The NovaLisa^® Toxoplasma gondii IgG Avidity Test is an additional reagent-kit that has to be used in combination with NovaLisa® Toxoplasma gondii IgG ELISA.

Performance Characteristics:

The NovaLisa® Toxoplasma gondii IgG Avidity Test has been evaluated for use in Toxoplasmosis with samples of acute and past infections. A total number of 86 defined patient samples were tested. These samples were supplied by the Institute of Medical Microbiology, Immunology and Parasitology, University Bonn.

		Defined Samples
		Acute Infection	Past Infection	Total	Agreement (%)
NovaLisa® Toxoplasma gondii IgG Avidity Test	Acute Infection	36	1	37	94.7
	Past Infection	2	47	49	97.9
	Total	38	48	86	96.5

Order information:

ELISA	Number of Determinations	Product Number
Toxoplasma IgG	96	TOXG0460
Toxoplasma IgM µ-capture	96	TOXM0460
Toxoplasma IgG Avidity Test*	96	TOXGA460

* The NovaLisa^® Toxoplasma gondii IgG Avidity Test is an additional reagent that has to be used in combination with the NovaLisa^® Toxoplama gondii IgG ELISA.

RiliBÄK

With Liquickeck^® ToRCH Plus Control (Bio-Rad) an external control is available for the NovaLisa^® Toxoplasma gondii IgG