詳細(xì)介紹
S3細(xì)胞分選儀是一款自動(dòng)化的臺(tái)式細(xì)胞分選儀,帶有1-2個(gè)激光器和*多4個(gè)熒光檢測(cè)器,以及正向和側(cè)向散射光檢測(cè)器。它利用*的ProDrop™技術(shù)進(jìn)行自動(dòng)化的液滴延遲計(jì)算和斷裂監(jiān)控,從而簡(jiǎn)化了細(xì)胞分選中*復(fù)雜的部分。這一高性價(jià)比的細(xì)胞分選儀提供了**的分選純度和結(jié)果,而不會(huì)折損其性能和靈敏度。此系統(tǒng)適用于分選表達(dá)熒光蛋白(如GFP)或帶有熒光標(biāo)記的細(xì)胞。
S3細(xì)胞分選儀為研究人員帶來(lái)了:
• 簡(jiǎn)化的儀器設(shè)置 – 自動(dòng)的液滴延遲計(jì)算和斷裂監(jiān)控實(shí)現(xiàn)了**的1-4色分選,而所需的培訓(xùn)極少
• 極少的交叉污染 – 雙位置上樣臺(tái)確保不同分選運(yùn)行之間的樣品線是干凈的
• 小巧的設(shè)計(jì) – 尺寸僅為2.3 x 2.1 x 2.1英尺,包括內(nèi)置的流體設(shè)備和溫度控制系統(tǒng)
• 直觀的界面 – 用戶友好的軟件帶來(lái)輕松的儀器控制和分選邏輯定義
Acquisition rate, events/sec Up to 100,000 Sorting rate, events/sec Up to 30,000 Sorting purity >99% pure Nozzle size, µm 100 Sorting direction 2-way sorting Sorting collection Up to 5 x 5 ml sample tubes each direction
Microscope slides
8-well strip each direction Lasers Primary: 488 nm 100 mW
Optional: 561 nm 100 mW Detection Forward scatter (FSC) with PMT
Side scatter (SSC) with PMT
Up to 4 fluorescent detectors with PMT
Minimum resolution: 0.5 µm Sensitivity, MESF <125 for FITC and PE Temperature range, °C Sample and collection temperature control system: 4–37
Peltier solid state system Fluidics Onboard fluidics and dilution of 8x sheath fluid with deionized water Data format FCS 3.1 Dimensions (W x D x H), ft/cm 2.3 x 2.1 x 2.1 / 70 x 65 x 65