植物維生素D(VD)ELISA 檢測試劑盒,植物維生素D(VD)ELISA 檢測試劑盒
植物維生素D(VD)ELISA 檢測試劑盒*試驗原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進行檢測。先將ZR和生物素標(biāo)記的抗體同時溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
植物維生素D(VD)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS11682B 大鼠血管內(nèi)皮細胞生長因子B(VEGF-B)elisa試劑盒 Rat Vascular Endothelial cell Growth Factor B,VEGF-B Elisa試劑盒
RS11683B 大鼠白介素12(IL-12/P40)elisa試劑盒 Rat Interleukin 12,IL-12/P40 Elisa試劑盒
RS11684B 大鼠血管內(nèi)皮細胞生長因子C(VEGF-C)elisa試劑盒 Rat Vascular Endothelial cell Growth Factor C,VEGF-C Elisa試劑盒
RS11685B 大鼠白介素11(IL-11)elisa試劑盒 Rat Interleukin 11,IL-11 Elisa試劑盒
RS11686B 大鼠胰島素樣生長因子結(jié)合蛋白4(IGFBP-4)elisa試劑盒 Rat Insulin-like growth factor binding protein 4,IGFBP-4 Elisa試劑盒
RS11687B 大鼠胰島素樣生長因子結(jié)合蛋白3(IGFBP-3)elisa試劑盒 Rat Insulin-like growth factor binding protein 3,IGFBP-3 Elisa試劑盒
RS11688B 大鼠血管內(nèi)皮細胞生長因子D(VEGF-D)elisa試劑盒 Rat Vascular Endothelial cell Growth Factor D,VEGF-D Elisa試劑盒
RS11689B 大鼠胰島素樣生長因子結(jié)合蛋白2(IGFBP-2)elisa試劑盒 Rat Insulin-like growth factor binding protein 2,IGFBP-2 Elisa試劑盒
RS11690B 大鼠血管內(nèi)皮細胞生長因子受體1(VEGFR-1)elisa試劑盒 Rat Vascuoar endothelial cell growth factor receptor 1,VEGFR-1 Elisa試劑盒
RS11691B 大鼠胰島素樣生長因子結(jié)合蛋白1(IGFBP-1)elisa試劑盒 Rat Insulin-like growth factor binding protein 1,IGFBP-1 Elisa試劑盒
RS11692B 大鼠血管內(nèi)皮細胞生長因子受體2(VEGFR-2)elisa試劑盒 Rat Vascuoar endothelial cell growth factor receptor 2,VEGFR-2 Elisa試劑盒
RS11693B 大鼠肝細胞生長因子(HGF)elisa試劑盒 Rat hepatocyte growth factor,HGF Elisa試劑盒
RS11694B 大鼠生長因子(GH)elisa試劑盒 Rat growth hormone,GH Elisa試劑盒
RS11695B 大鼠堿性成纖維細胞生長因子9(bFGF-9)elisa試劑盒 Rat basic fibroblast growth factor 9,bFGF-9 Elisa試劑盒
RS11696B 大鼠堿性成纖維細胞生長因子4(bFGF-4)elisa試劑盒 Rat basic fibroblast growth factor 4,bFGF-4 Elisa試劑盒
RS11697B 大鼠堿性成纖維細胞生長因子6(bFGF-6)elisa試劑盒 Rat basic fibroblast growth factor 6,bFGF-6 Elisa試劑盒
RS11698B 大鼠酸性成纖維細胞生長因子1(aFGF-1)elisa試劑盒 Rat acidic fibroblast growth factor 1,aFGF-1 Elisa試劑盒
RS11699B 大鼠凋亡相關(guān)因子配體(FASL) elisa試劑盒 Rat Factor-related Apoptosis ligand,FASL Elisa試劑盒
RS11700B 大鼠凋亡相關(guān)因子(FAS/CD95)elisa試劑盒 Rat Factor-related Apoptosis,FAS Elisa試劑盒
RS11701B 大鼠紅細胞生成素(EPO)elisa試劑盒 Rat Erythropoietin,EPO Elisa試劑盒
RS11702B 大鼠紅細胞生成素受體(EPOR)elisa試劑盒 Rat Erythropoietin receptor,EPOR Elisa試劑盒
RS11703B 大鼠嗜酸粒細胞趨化蛋白Eotaxin 1(Eotaxin 1/CCL11)elisa試劑盒 Rat Eotaxin 1 Elisa試劑盒 ab12212 Mouse monoclonal [AZ44] to Polo like kinase (phospho) 100ug 3450
ab12213 Goat polyclonal to c-Myc (HRP) 50ug 3450
ab12214 Mouse monoclonal to Treponema pallidum 100ug 3450
ab12217 Mouse monoclonal [CSL1-5] to Calpastatin 50ug 3450
ab12218 Mouse monoclonal [CSL5-10] to Calpastatin 50ug 3450
ab12219 Mouse monoclonal [BU6-4] to BrdU 50ug 3450
ab1222 Rabbit polyclonal to SOD 1ml 3450
ab12220 Mouse monoclonal [GTS-1] to Heme Oxygenase 1 50ug 3450
ab12221 Rabbit polyclonal to N Cadherin 100ug 3795
ab12222 Mouse monoclonal [NCC-CAD-299] to P cadherin 50ug 3450
ab12223 Mouse monoclonal [10C3] to KDEL - ER Marker 100ug 3450
ab12224 Mouse monoclonal [KR-10] to KDEL Receptor 50ul 3450
ab12225 Mouse monoclonal [1D3] to PDI - ER Marker 100ug 3450
ab12228 Mouse monoclonal [UC1MT] to Metallothionein 50ug 3450
ab1223 Rabbit polyclonal to Transferrin 1ml 3450
ab12233 Rabbit polyclonal to Calcineurin A 50ug 3450
ab1224 Rabbit polyclonal to Cyclin D1 100ul 3450
ab12241 Rabbit polyclonal to Secretogranin II 100ul 3450
ab12242 Rabbit polyclonal to Chromogranin B 100ul 3450
ab12243 Chicken polyclonal to Raspberry Bushy Dwarf Virus Coat Protein 125ug 3450
ab12244 Chicken polyclonal to Tomato Spotted Wilt Virus Core Protein 125ug 3450
ab12246 Mouse monoclonal to Staphylococcus Enterotoxin A 100ug 3450
ab12248 Mouse monoclonal to Lipoteichoic Acid 500ug 3450
ab12249 Rabbit polyclonal to TRPC6 50ul 3450
ab1225 Mouse monoclonal [BB12 + CB19] to CD3 + CD19 (Dual color: FITC + PE) 500ul 3450
ab12251 Mouse monoclonal to Beta Lactamase 500ug 3450
ab12252 Mouse monoclonal to Beta Lactamase 500ug 3450
ab12253 Rabbit polyclonal to Respiratory Syncytial Virus 50ug 3450
ab12254 Mouse monoclonal [AS61] to EDG3 25ug 3450
ab12258 Rabbit polyclonal to cGKI 50ul 3450
ab1226 Mouse monoclonal [BB12 + LN3] to CD3 + HLA-DR (Dual color: FITC + PE) 500ul 3450
ab12261 Rabbit polyclonal to Nurr1 100ug 3450
ab12265 Rabbit polyclonal to beta Amyloid 1-40 100ul 3450
ab12266 Mouse monoclonal [DE2B4] to Amyloid Precursor Protein 1ml 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實驗中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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