大鼠雌三醇(E3)ELISA 檢測(cè)試劑盒,大鼠雌三醇(E3)ELISA 檢測(cè)試劑盒
大鼠雌三醇(E3)ELISA 檢測(cè)試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡(jiǎn)單介紹】
大鼠雌三醇(E3)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS13061B 小鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)elisa試劑盒 Mouse plaet membrane glycoprotein ⅡbⅢa,GP-ⅡbⅢa Elisa試劑盒
RS13062B 小鼠游離脂肪酸(FFA)elisa試劑盒 Mouse free fatty acids,FFA Elisa試劑盒
RS13063B 小鼠血小板反應(yīng)蛋白/凝血酶敏感蛋白1(TSP-1)elisa試劑盒 Mouse thrombospondin 1,TSP-1 Elisa試劑盒
RS13064B 小鼠骨粘連蛋白(ON)elisa試劑盒 Mouse osteonectin,ON Elisa試劑盒
RS13065B 小鼠葉酸(FA)elisa試劑盒 Mouse Folic acid,FA Elisa試劑盒
RS13066B 小鼠組蛋白H2b(histon-H2b)elisa試劑盒 Mouse histon-H2b Elisa試劑盒
RS13067B 小鼠糖皮質(zhì)類固醇受體(GR)elisa試劑盒 Mouse glucocorticoid receptor,GR Elisa試劑盒
RS13068B 小鼠黃體生成素釋放激素(LHRH)elisa試劑盒 Mouse Luteinizing Hormone-Releasing Hormone,LHRH Elisa試劑盒
RS13069B 小鼠腦紅蛋白(NGB)elisa試劑盒 Mouse Neuroglobin,NGB Elisa試劑盒
RS13070B 小鼠8異前列腺素(8-iso-PG)elisa試劑盒 Mouse 8-iso prostaglandin,8-iso-PG Elisa試劑盒
RS13071B 小鼠γ干擾素誘導(dǎo)蛋白16/p16(IFI16/p16)elisa試劑盒 Mouse interferon-inducible protein 16,IFI16/p16 Elisa試劑盒
RS13072B 小鼠基質(zhì)細(xì)胞衍生因子1a(SDF-1a/CXCL12)elisa試劑盒 Mouse Stromal cell derived factor 1a,SDF-1a Elisa試劑盒
RS13073B 小鼠血漿α顆粒膜蛋白(GMP-140)elisa試劑盒 Mouse alpha-granular membrane protein,GMP-140 Elisa試劑盒
RS13074B 小鼠髓過氧化物酶(MPO)elisa試劑盒 Mouse myeloperoxidase,MPO Elisa試劑盒
RS13075B 小鼠顆粒酶B(Gzms-B)elisa試劑盒 Mouse granzymes B,Gzms-B Elisa試劑盒
RS13076B 小鼠顆粒酶A(Gzms-A)elisa試劑盒 Mouse granzymes A,Gzms-A Elisa試劑盒
RS13077B 小鼠淋巴細(xì)胞趨化因子(Lptn/LTN/XCL1)elisa試劑盒 Mouse lymphotactin,Lptn/LTN Elisa試劑盒
RS13078B 小鼠末端補(bǔ)體復(fù)合物C5b-9(TCC C5b-9)elisa試劑盒 Mouse terminal complement complex C5b-9,TCC C5b-9 Elisa試劑盒
RS13079B 小鼠補(bǔ)體蛋白4(C4)elisa試劑盒 Mouse Complement 4,C4 Elisa試劑盒ab27075 Mouse monoclonal [TU-30] to gamma Tubulin (Dyomics 547) 100ug 3450
ab27076 Mouse monoclonal [TU-30] to gamma Tubulin (Dyomics 647) 100ug 3450
ab27077 Rabbit polyclonal to Munc 13 100ug 3450
ab27085 Rabbit polyclonal to NRG1, prediluted 7ml 3450
ab27086 Rabbit polyclonal to ErbB 2 500ul 3450
ab27087 Rabbit polyclonal to CD5 7ml 3450
ab27089 Rabbit polyclonal to CD8 7ml 3450
ab27090 Rabbit polyclonal to CD79a 7ml 3450
ab27091 Rabbit polyclonal to CD95 , prediluted 7ml 3450
ab27092 Rabbit polyclonal to CD20, prediluted 7ml 3450
ab27093 Rabbit polyclonal to CD20 500ul 3450
ab27094 Rabbit polyclonal to Androgen Receptor, prediluted 7ml 3450
ab2710 Mouse monoclonal [LN2] to CDw74 500ul 3450
ab27102 Goat polyclonal to Munc 13 100ug 2826
ab2712 Mouse monoclonal [TIA-1] to TIA1 250ul 3450
ab2714 Goat polyclonal to UEV1 (221aa isoform b) 100ug 3450
ab27141 Mouse monoclonal [spm139] to Insulin, prediluted 7ml 3450
ab27144 Rabbit polyclonal to Placental alkaline phosphatase (PLAP), prediluted 7ml 3450
ab27147 Rabbit polyclonal to Caspase 7 500ul 3450
ab27148 Mouse monoclonal [HYB266-02] to Simazine/Atrazine 100ug 3450
ab27149 Mouse monoclonal [HYB269-01] to Cyanazine 100ug 3450
ab2715 Goat polyclonal to RGS1 100ug 3450
ab27150 Mouse monoclonal [HYB276-02] to Triazines 100ug 3450
ab27151 Mouse monoclonal [HYB283-02] to Hydroxytriazine 100ug 3450
ab27152 Mouse monoclonal [HYB273-01] to 2,6-dichlorobenzamide 100ug 3450
ab27153 Mouse monoclonal [HYB337-01] to S-Mecoprop 100ug 3450
ab27154 Mouse monoclonal [HYB337-02] to S-Mecoprop 100ug 3450
ab27155 Mouse monoclonal [HYB332-01] to beta 2 Microglobulin 100ug 3450
ab27156 Mouse monoclonal [HYB331-01] to ds DNA 100ug 3450
ab27157 Mouse monoclonal [HYB330-01] to MAP kinase substrate 1 100ug 3450
ab27158 Mouse monoclonal [20.6] to Bordela pertussis toxin subunit S2 100ug 3450
ab27159 Mouse monoclonal [21.3D11] to Bordela pertussis toxin subunit S3 100ug 3450
ab2716 Goat polyclonal to Melanophilin 100ug 3450
ab27160 Mouse monoclonal [HYB339-01] to alpha 2 Microglobulin 100ug 3450
ab27161 Mouse monoclonal [HYB339-02] to alpha 2 Microglobulin 100ug 3450
ab27162 Mouse monoclonal [HYB338-01] to Tau 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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