大鼠P53(P53)ELISA 檢測試劑盒,大鼠P53(P53)ELISA 檢測試劑盒
大鼠P53(P53)ELISA 檢測試劑盒*試驗(yàn)原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對(duì)照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
大鼠P53(P53)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12861B 小鼠血管內(nèi)皮細(xì)胞生長因子受體-3(VEGFR-3)elisa試劑盒 Mouse Vascuoar endothelial cell growth factor receptor,VEGFR-3 Elisa試劑盒
RS12862B 小鼠血管內(nèi)皮細(xì)胞生長因子受體2(VEGFR-2)elisa試劑盒 Mouse Vascuoar endothelial cell growth factor receptor 2,VEGFR-2 Elisa試劑盒
RS12863B 小鼠血管內(nèi)皮細(xì)胞生長因子受體1(VEGFR-1)elisa試劑盒 Mouse Vascuoar endothelial cell growth factor receptor 1,VEGFR-1 Elisa試劑盒
RS12864B 小鼠血管內(nèi)皮細(xì)胞生長因子(VEGF)elisa試劑盒 Mouse Vascular Endothelial cell Growth Factor,VEGF Elisa試劑盒
RS12865B 小鼠血管內(nèi)皮細(xì)胞粘附分子1(VCAM-1/CD106)elisa試劑盒 Mouse Vascuolar cell adhesion molecule 1,VCAM-1 Elisa試劑盒
RS12866B 小鼠血小板生成素(TPO)elisa試劑盒 Mouse Thrombopoietin,TPO Elisa試劑盒
RS12867B 小鼠腫瘤壞死因子β(TNF-β)elisa試劑盒 Mouse Tumor necrosis factor β,TNF-β Elisa試劑盒
RS12868B 小鼠腫瘤壞死因子α(TNF-α)elisa試劑盒 Mouse Tumor necrosis factor α,TNF-α Elisa試劑盒
RS12869B 小鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)elisa試劑盒 Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ Elisa試劑盒
RS12870B 小鼠腫瘤壞死因子可溶性受體Ⅰ(TNFsR-Ⅰ)elisa試劑盒 Mouse Tumor necrosis factor soluble receptor Ⅰ,TNFsR-Ⅰ Elisa試劑盒
RS12871B 小鼠轉(zhuǎn)化生長因子β1(TGF-β1)elisa試劑盒 Mouse Transforming Growth factor β1,TGF-β1 Elisa試劑盒
RS12872B 小鼠基質(zhì)細(xì)胞衍生因子1β(SDF-1β/CXCL12)elisa試劑盒 Mouse Stromal cell derived factor 1β,SDF-1β Elisa試劑盒
RS12873B 小鼠干細(xì)胞因子/肥大細(xì)胞生長因子(SCF/MGF)elisa試劑盒 Mouse Stem cell factor/mast cell growth factor,SCF/MGF Elisa試劑盒
RS12874B 小鼠可溶性白細(xì)胞分化抗原40配體(sCD40L)elisa試劑盒 Mouse Soluble Cluster of differentiation 40 ligand,sCD40L Elisa試劑盒
RS12875B 小鼠P選擇素(P-Selectin/CD62P)elisa試劑盒 Mouse P-Selectin Elisa試劑盒
RS12876B 小鼠血小板衍生生長因子AB(PDGF-AB)elisa試劑盒 Mouse Plaet-Derived Growth Factor AB,PDGF-AB Elisa試劑盒
RS12877B 小鼠血小板衍生生長因子可溶性受體α(PDGFsR-α)elisa試劑盒 Mouse Plaet-Derived Growth Factor Soluble Receptor α,PDGFsR-α Elisa試劑盒
RS12878B 小鼠抑瘤素M(OSM)elisa試劑盒 Mouse Oncostatin-M,OSM Elisa試劑盒
RS12879B 小鼠B因子(BF)elisa試劑盒 Mouse factor B,BF Elisa試劑盒
RS12880B 小鼠骨保護(hù)素(OPG)elisa試劑盒 Mouse Osteoprotegerin,OPG Elisa試劑盒
RS12881B 小鼠神經(jīng)營養(yǎng)因子4(NT-4)elisa試劑盒 Mouse Neurotrophin 4,NT-4 Elisa試劑盒 ab2944 Rabbit polyclonal to p19 INK4d 500ul 3450
ab2946 Rabbit polyclonal to p18 INK4c 500ul 3450
ab2947 Rabbit polyclonal to p27 KIP 1 500ul 3450
ab2948 Rabbit polyclonal to Cyclin A 500ul 3450
ab2949 Rabbit polyclonal to Cyclin B1 500ul 3450
ab2950 Rabbit polyclonal to Cyclin C 500ul 3450
ab2952 Rabbit polyclonal to Cdk2 500ul 3450
ab2953 Rabbit polyclonal to Cdk3 500ul 3450
ab2954 Rabbit polyclonal to Cdk6 500ul 3450
ab2955 Rabbit polyclonal to Cdk8 500ul 3450
ab2956 Rabbit polyclonal to BRCA1 500ul 3450
ab2957 Rabbit polyclonal to BRCA2 500ul 3450
ab2958 Rabbit polyclonal to Nck 500ul 3450
ab2960 Rabbit polyclonal to E2F1 500ul 3450
ab2961 Rabbit polyclonal to p21 500ul 3450
ab2962 Rabbit polyclonal to Thrombospondin 500ul 3450
ab2964 Rabbit polyclonal to Cullin 1 500ul 3450
ab2965 Rabbit polyclonal to Cdc34 500ul 3450
ab2967 Rabbit polyclonal to Cullin 2 500ul 3450
ab2968 Rabbit polyclonal to Cyclin E2 500ul 3450
ab2969 Rabbit polyclonal to DNA Polymerase gamma 500ul 3450
ab2970 Rabbit polyclonal to Bag1 500ul 3450
ab2971 Rabbit polyclonal to SIRP alpha 500ul 3450
ab2972 Rabbit polyclonal to Transglutaminase 2 500ul 3450
ab2974 Rabbit polyclonal to ErbB 3 500ul 3450
ab2976 Rabbit polyclonal to Apc2 500ul 3450
ab2977 Rabbit polyclonal to ROC1 500ul 3450
ab2978 Rabbit polyclonal to MMP11 500ul 3450
ab2980 Rabbit polyclonal to Endostatin 500ul 3450
ab2981 Rabbit polyclonal to alpha 1 Catenin 500ul 3450
ab29818 Pancreas (Human) Whole Cell Lysate - fetal normal tissue 500ug 1725
ab2982 Rabbit polyclonal to beta Catenin 500ul 3450
ab2983 Rabbit polyclonal to STAT1 500ul 3450
ab2984 Rabbit polyclonal to STAT3 500ul 3450
ab2987 Rabbit polyclonal to Topoisomerase II alpha 500ul 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請(qǐng)盡快用水沖洗。
2. 實(shí)驗(yàn)中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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