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血管內(nèi)皮生長(zhǎng)因子A(VEGFA)檢測(cè)試劑盒

參考價(jià)	￥2008.00

參考價(jià)

￥2008.00

具體成交價(jià)以合同協(xié)議為準(zhǔn)

公司名稱杭州昊鑫生物科技股份有限公司
品牌云克隆
型號(hào)SEA143Ca
所在地杭州市
廠商性質(zhì)代理商
更新時(shí)間2024/7/11 14:36:50
訪問次數(shù)136

產(chǎn)品標(biāo)簽:

酶聯(lián)免疫吸附試驗(yàn)法

規(guī)格

48T

2008.00元

10000 盒可售

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展開顯示更多內(nèi)容

胎牛血清、MCE抑制劑激動(dòng)劑、RNA提取試劑盒、ELISA試劑盒、重組蛋白

產(chǎn)品簡(jiǎn)介在線詢價(jià)

產(chǎn)地	進(jìn)口	規(guī)格	48T
級(jí)別	化工級(jí)	證書	ISO系列證書

本試劑盒運(yùn)用雙抗體夾心 ELISA 法定量測(cè)定犬血清、血漿、組織勻漿、細(xì)胞裂解液、細(xì)胞培養(yǎng)上清或其它相關(guān)生物液體中 VEGFA 含量。

血管內(nèi)皮生長(zhǎng)因子A(VEGFA)檢測(cè)試劑盒產(chǎn)品信息

血管內(nèi)皮生長(zhǎng)因子A(VEGFA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

編號(hào)SEA143Ca
物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
實(shí)驗(yàn)方法雙抗夾心
反應(yīng)時(shí)長(zhǎng)3h
檢測(cè)范圍15.6-1,000pg/mL
靈敏度最小可檢測(cè)劑量小于等于5.6pg/mL.
樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
下載英文說明書中文說明書
規(guī)格48T96T 96T*5 96T*10 96T*100
價(jià)格￥ 2008

產(chǎn)品包裝圖模擬

特異性

本試劑盒用于檢測(cè)血管內(nèi)皮生長(zhǎng)因子A(VEGFA)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本	回收率范圍(%)	平均回收率(%)
serum(n=5)	88-95	92
EDTA plasma(n=5)	83-93	88
heparin plasma(n=5)	88-102	91

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中血管內(nèi)皮生長(zhǎng)因子A(VEGFA)含量的測(cè)定值與理論值的比率。

樣本	1:2	1:4	1:8	1:16
serum(n=5)	79-102%	79-103%	94-103%	79-97%
EDTA plasma(n=5)	89-98%	93-104%	90-98%	83-98%
heparin plasma(n=5)	84-91%	78-88%	78-98%	87-105%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將血管內(nèi)皮生長(zhǎng)因子A(VEGFA)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)與連接于固相載體上的抗體結(jié)合，然后加入生物-素化的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)抗體，將未結(jié)合的生物-素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管內(nèi)皮生長(zhǎng)因子A(VEGFA)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

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