Protein A Agarose
蛋白 A 瓊脂糖
MCE Protein A Agarose 以高度交聯(lián)的 4% 瓊脂糖凝膠為基質，通過化學方法定向、高效偶聯(lián)了重組 Protein A，可從動物腹水、血清、細胞分泌液上清等生物體液中一步分離、純化 IgG 等。
Description& Advantages
Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus and binds to most mammalian IgGs mainly through Fc regions. Native Protein A contains 5 IgG binding domains and many other domains with unknown functions. The latter have been eliminated from recombinant Protein A to reduce nonspecific binding.
MCE Protein A Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein A, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG, IgE, IgM.
| Characteristics | |
|---|---|
| Matrix Spherical | 4% cross-linked agarose |
| Degree of Substitution | 2 mg Recombinant Protein A/mL Settled Resin |
| Bead Diameter | 45-165 μm |
| Binding Capacity | 20 mg human IgG/mL Settled Resin |
| Storage Solution | 50% slurry in 1× PBS containing 20% ethanol |
Storage
Store at 2-8oC, and is stable for at least 2 years.
Do not dry or freeze.
注：產品僅用于科研
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