Texas Red (Synonyms: 磺基羅丹明 101; Sulforhodamine 101; SR101)
Texas Red (Sulforhodamine 101) 是一種兩性羅丹明紅色熒光染料 (excitation/emission: 586/605 nm)。Texas Red 被廣用于研究神經(jīng)元形態(tài)，并作為星形膠質(zhì)細(xì)胞的細(xì)胞類型選擇性熒光標(biāo)記物，在體內(nèi)和切片制備中都有應(yīng)用。
生物活性
Texas Red (Sulforhodamine 101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Texas Red is used extensively for investigating neuronal morphology and acts as a cell type-selective fluorescent marker of astrocytes both in vivo and in slice preparations[1].
體外研究(In Vitro)
Texas Red (Sulforhodamine 101) does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Texas Red has to be kept as low as possible or the labeling procedure can be performed after the actual experiment[1].
體內(nèi)研究(In Vivo)
In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Texas Red or topical application of 100 μM[1].
分子量：606.71
Formula：C31H30N2O7S2
CAS 號：60311-02-6
中文名稱：磺基羅丹明101
運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.
儲存方式：
-20°C, protect from light
*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。
溶解性數(shù)據(jù)
In Vitro:
DMSO : 50 mg/mL (82.41 mM; Need ultrasonic)
H2O : 8.33 mg/mL (13.73 mM; Need ultrasonic)
| 濃度溶劑體積質(zhì)量 | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.6482 mL | 8.2412 mL | 16.4823 mL |
| 5 mM | 0.3296 mL | 1.6482 mL | 3.2965 mL |
| 10 mM | 0.1648 mL | 0.8241 mL | 1.6482 mL |
請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。
以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶
- 1.
請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.08 mg/mL (3.43 mM); Suspended solution; Need ultrasonic
- 2.
請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (3.43 mM); Suspended solution; Need ultrasonic
參考文獻(xiàn)
[1]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9. [Content Brief]
注：產(chǎn)品僅用于科研
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