肺炎支原體(MP)IGM檢測試劑盒(血清.血漿.組織)齊一生物長期經(jīng)營ELISA試劑盒,進(jìn)口ELISA試劑盒及抗體(免費(fèi)代測),分子學(xué)生物試劑盒,金標(biāo)法檢測試劑盒,比色法檢測試劑盒,細(xì)胞菌株,培養(yǎng)基,細(xì)胞因子,生化試劑,耗材等生物試劑產(chǎn)品.誠信經(jīng)營,價(jià)格實(shí)惠,服務(wù)周到,質(zhì)量保障!提供各種實(shí)驗(yàn)代測服務(wù).歡迎新老客戶來電齊一生物科技(上海)有限公司,專業(yè)生產(chǎn)研發(fā)生化科研試劑:
肺炎支原體(MP)IGM檢測試劑盒(血清.血漿.組織)制備方法
包括以下步驟:
1)抗原表位的計(jì)算機(jī)篩選;
2)抗原的制備;
3)血清的采集;
4)間接ELISA方法的建立;
5)間接ELISA方法的敏感性和特異性驗(yàn)證;
6)試劑盒的穩(wěn)定性驗(yàn)證;
7)對(duì)屠宰場進(jìn)行臨床檢測.該方法簡單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測,可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清:操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿:EDTA、檸檬酸鹽、肝素血漿可用于檢測.1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液:1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿:將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存:如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
SignalSilence? EWS siRNA I 12175S 美國Cell Signaling Technology(CST)
Phospho-Akt (Thr450) (D5G4) Rabbit mAb 12178S 美國Cell Signaling Technology(CST)
EAAT3 Antibody 12179S 美國Cell Signaling Technology(CST)
BRD4 Antibody 12183S 美國Cell Signaling Technology(CST)
p27 Kip1 (D69C12) XP? Rabbit mAb (PE Conjugate) 12184S 美國Cell Signaling Technology(CST)
PhosphoPlus? PERK (Thr980) Antibody Duet 12185S 美國Cell Signaling Technology(CST)
Bim (C34C5) Rabbit mAb (PE Conjugate) 12186S 美國Cell Signaling Technology(CST)
RNF40 (D2R2O) Rabbit mAb 12187S 美國Cell Signaling Technology(CST)
c-Myc (D84C12) XP? Rabbit mAb (PE Conjugate) 12189S 美國Cell Signaling Technology(CST)
SPT16 (D7I2K) Rabbit mAb 12191S 美國Cell Signaling Technology(CST)
PSMB7 Antibody 12197S 美國Cell Signaling Technology(CST)
CCR2 (D14H7) Rabbit mAb 12199S 美國Cell Signaling Technology(CST)
PhosphoPlus? Histone H3 (Ser10) Antibody Duet 12201S 美國Cell Signaling Technology(CST)
Ki-67 (D3B5) Rabbit mAb (Mouse Specific; IHC Formulated) 12202S 美國Cell Signaling Technology(CST)
PKCθ (P632) Antibody 12206S 美國Cell Signaling Technology(CST)
MAPK Family Alexa Fluor? 647 Conjugated Antibody Sampler Kit 12207S 美國Cell Signaling Technology(CST)
Nilotinib 12209S 美國Cell Signaling Technology(CST)
Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide 1220S 美國Cell Signaling Technology(CST)
FKBP5 (D5G2) Rabbit mAb 12210S 美國Cell Signaling Technology(CST)
Rab3A (D7B10) Rabbit mAb 12214S 美國Cell Signaling Technology(CST)
TREX1 Antibody 12215S 美國Cell Signaling Technology(CST)
SignalSilence? EWS siRNA II 12216S 美國Cell Signaling Technology(CST)
SHANK2 Antibody 12218S 美國Cell Signaling Technology(CST)
Phospho-eIF2α (Ser51) Blocking Peptide 1221S 美國Cell Signaling Technology(CST)
Tenascin C (D16C4) Rabbit mAb 12221S 美國Cell Signaling Technology(CST)
Mouse IL-3 Neutralizing (D6C1) Rabbit mAb 12222S 美國Cell Signaling Technology(CST)
FLI1 Antibody 12225S 美國Cell Signaling Technology(CST)
HMOX2/HO-2 Antibody 12226S 美國Cell Signaling Technology(CST)
IL-4 (D19A10) Rabbit mAb 12227S 美國Cell Signaling Technology(CST)
SignalSilence? Sec24B siRNA I 12229S 美國Cell Signaling Technology(CST)
Histone H3 (D1H2) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate) 12230S 美國Cell Signaling Technology(CST)
Cyclin B1 (D5C10) XP? Rabbit mAb 12231P 美國Cell Signaling Technology(CST)
Cyclin B1 (D5C10) XP? Rabbit mAb 12231S 美國Cell Signaling Technology(CST)
PTMScan? Mono-Methyl Arginine Motif [mme-RG] Kit 12235S 美國Cell Signaling Technology(CST)
Syndecan 4 Antibody 12236S 美國Cell Signaling Technology(CST)
Calreticulin (D3E6) XP? Rabbit mAb 12238P 美國Cell Signaling Technology(CST)
Calreticulin (D3E6) XP? Rabbit mAb 12238S 美國Cell Signaling Technology(CST)
IL-2 (D7A5) Rabbit mAb 12239S 美國Cell Signaling Technology(CST)
SignalSilence? SirT1 siRNA I 12241S 美國Cell Signaling Technology(CST)
IL-1β (3A6) Mouse mAb 12242S 美國Cell Signaling Technology(CST)
Drebrin A Antibody 12243S 美國Cell Signaling Technology(CST)
Di-Methyl-Histone H3 (Lys27) (D18C8) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate) 12244S 美國Cell Signaling Technology(CST)
TPX2 (D2R5C) XP? Rabbit mAb 12245P 美國Cell Signaling Technology(CST)
TPX2 (D2R5C) XP? Rabbit mAb 12245S 美國Cell Signaling Technology(CST)
Nucleolin Antibody 12247S 美國Cell Signaling Technology(CST)
Skp1 (D3J4N) Rabbit mAb 12248S 美國Cell Signaling Technology(CST)
CYP3A4 Antibody 12249S 美國Cell Signaling Technology(CST)
Ambra1 Antibody 12250S 美國Cell Signaling Technology(CST)
Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb 12251S 美國Cell Signaling Technology(CST)
CPT1A (D3B3) Rabbit mAb 12252S 美國Cell Signaling Technology(CST)
SignalSilence? Eps15 siRNA I 12253S 美國Cell Signaling Technology(CST)
Plectin-1 (D6A11) Rabbit mAb 12254S 美國Cell Signaling Technology(CST)
Lamin B2 (D8P3U) Rabbit mAb 12255S 美國Cell Signaling Technology(CST)
SignalSilence? c-Raf siRNA I 12256S 美國Cell Signaling Technology(CST)
E2A (D2B1) Rabbit mAb 12258S 美國Cell Signaling Technology(CST)
TAP2 Antibody 12259S 美國Cell Signaling Technology(CST)
Bcl-xL Blocking Peptide 1225S 美國Cell Signaling Technology(CST)
β-Actin (8H10D10) Mouse mAb (HRP Conjugate) 12262S 美國Cell Signaling Technology(CST)
G6PD (D5D2) Rabbit mAb 12263S 美國Cell Signaling Technology(CST)
TH1L (D5G6W) Rabbit mAb 12265S 美國Cell Signaling Technology(CST)
PTMScan? Phospho-ATM/ATR Substrate Motif [pSQ] Kit 12267S 美國Cell Signaling Technology(CST)
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb (PE Conjugate) 12268S 美國Cell Signaling Technology(CST)
Synaptophysin (7H12) Mouse mAb 12270S 美國Cell Signaling Technology(CST)
β1-Adrenergic Receptor Antibody 12271S 美國Cell Signaling Technology(CST)
MDR1/ABCB1 Antibody 12273S 美國Cell Signaling Technology(CST)
IFN-γ (D3H2) XP? Rabbit mAb (PE Conjugate) 12274S 美國Cell Signaling Technology(CST)
MAG (D10H1) Rabbit mAb 12275S 美國Cell Signaling Technology(CST)
Toll-like Receptor 2 (D7G9Z) Rabbit mAb 12276S 美國Cell Signaling Technology(CST)
PACT Antibody 12277S 美國Cell Signaling Technology(CST)
Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb 12280S 美國Cell Signaling Technology(CST)
SimpleChIP? Human EP300 Promoter Primers 12281S 美國Cell Signaling Technology(CST)
Cox2 (D5H5) XP? Rabbit mAb 12282P 美國Cell Signaling Technology(CST)
Cox2 (D5H5) XP? Rabbit mAb 12282S 美國Cell Signaling Technology(CST)
CARD9 Antibody (Mouse Preferred) 12283S 美國Cell Signaling Technology(CST)
Maspin (mammary serine protease inhibitor) 抑癌基因(抗原)
MAG-a/b (Myelin associated glycoprotein; L / S -MAG;) 髓鞘相關(guān)糖蛋白a/b(抗原)
MAG (Myelin associated glycoprotein; L-MAG;MAG-a ) 髓鞘相關(guān)糖蛋白(抗原)
MAG-a (Myelin associated glycoprotein; L-MAG; ) 髓鞘相關(guān)糖蛋白-a(抗原)
MMP-1(Mous ematrix metalloproteinases-1) 基質(zhì)金屬蛋白酶-1(小鼠抗原)
midnolin isoform Protein 1 中腦核仁蛋白1(抗原)
midnolin isoform Protein 2 中腦核仁蛋白2(抗原)
MICA(MHC class I polypeptide-related sequence A) 一種細(xì)胞應(yīng)激分子抗原
MRP3(Multidrug Resistanec-Associated Protein 3) 多藥耐藥相關(guān)蛋白3(抗原)
MRP1(Multidrug Resistanec-Associated Protein 1) 多藥耐藥相關(guān)蛋白1(抗原)
LRP/MVP (Lung resistance related protein) 肺耐藥相關(guān)蛋白(抗原)
MSLN(mesothelin) 間皮素(多肽抗原)
MSH2 (mutS homolog 2) MSH2抗原
NKR(Neuromedin B Receptor) 神經(jīng)激肽B受體(抗原)
NF-L(Neurofilament triplet L) 低分子量神經(jīng)絲蛋白(抗原)
NF-M (Neurofilament triplet M) 中分子量神經(jīng)絲蛋白(抗原)
NF-H(Neurofilament triplet H) 高分子量神經(jīng)絲蛋白(抗原)
NIS(Na+/I-symporter;NIS) 鈉碘轉(zhuǎn)運(yùn)體蛋白(多肽抗原)
NFKBp65(p65 NF-kappa B;p65NFKB) 細(xì)胞核因子或稱k基因結(jié)合核因子(多肽抗原)
NR2A (Glutamate receptor2A) 谷氨酸受體(N端抗原)
NRP-1(neuropilin-1) 神經(jīng)纖毛蛋白-1(抗原)
NRP-2(neuropilin-2) 神經(jīng)纖毛蛋白-2(抗原)
NSBP1(Nucleosome-binding protein 1) 核小體結(jié)合蛋白1(抗原)
NTP,Neurual thread protein 神經(jīng)絲蛋白(抗原)
OAT-1 (Organic anion transporter 1)human 陰離子轉(zhuǎn)運(yùn)蛋白-1(人源)
OAT-1 (Organic anion transporter 1)mouse、rat 陰離子轉(zhuǎn)運(yùn)蛋白-1(大、小鼠同源:人源見RC-0606P)
OAT-3(Organic anion transporter 3) 陰離子轉(zhuǎn)運(yùn)蛋白-3(抗原)
UAT (Urate transporter/channel) 尿酸鹽轉(zhuǎn)運(yùn)子(抗原)
Oct-4 胚胎干細(xì)胞關(guān)鍵蛋白(多肽)
OPN(osteopontin) 骨橋蛋白(抗原)
P16/CDKN2A (Cyclin-dependent kinase inhibitor-2A) 抑癌基因p16(抗原)
p21/WAF1/CIP1 p21 核分裂抑制因子之一(抗原)
p27 (cyclin-dependent kinase inhibitor 1B) p27(抗原)
P63 protein P63 腫瘤抑制基因(抗原)
OPG(Osteoprotegerin) 護(hù)骨素(多肽抗原)
OPGL (Osteoprotegerin ligand) 骨保護(hù)蛋白配體(抗原)
P311 protein 增生性瘢痕相關(guān)蛋白(抗原)
PARP(poly ADP-ribose polymerase) 多腺苷二磷酸多聚酶抗原
Visfatin-1 (PBEF1)(NT) Human 內(nèi)脂素/內(nèi)臟脂肪素(人:N端多肽)、內(nèi)臟脂肪素、 又稱:前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素
Visfatin-1 (PBEF1)(NT) hu、 mo、 rat、MK 內(nèi)脂素/內(nèi)臟脂肪素(人、大、小鼠、猴同源:N端多肽)、內(nèi)臟脂肪素、前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素
Visfatin-2(CT)hu、mo、rat、MK 內(nèi)脂素/內(nèi)臟脂肪素(抗人、大、小鼠、猴:N端多肽)前B細(xì)胞克隆增強(qiáng)因子、內(nèi)肥素、腹脂素
PEDF (pigment epithelium-derived factor) 色素上皮源性因子(多肽抗原)
PAP2c (Phosphatidic acid phosphatase 2c) 磷脂酸磷酸酶2C(抗原)
PEPT1 (Peptide-transporters 1) 腸道肽轉(zhuǎn)運(yùn)蛋白(小肽轉(zhuǎn)運(yùn)蛋白)多肽
PTEN/MMAC1 一種腫瘤抑制基因抗原
PIBF(progesterone induced blocking factor) 孕激素誘導(dǎo)阻斷因子(抗原)
PIWIL1(piwi-like 1) piwi 樣1蛋白(抗原)
PPARγ (peroxisome proliferator-activated receptor-gamma) 過氧化酶活化增生受體γ(抗原)
PP2Ac 蛋白磷酸酶4(抗原)
Runx3 一種新發(fā)現(xiàn)的抑癌基因(抗原)
RRAD (Ras-related associated with diabetes ) RRAD(多肽抗原)
SDF-1/CXCL12 (stromal cell derived factor-1) 基質(zhì)細(xì)胞衍生因子-1(抗原)
shank1(SH3 and multiple anleyrin repead domins protein 1) shank1抗原
slc22A17 (solute carrier famili 22) 溶質(zhì)轉(zhuǎn)運(yùn)蛋白家族22成員17(多肽抗原)
SLC24A5 (solute carrier family 24, member 5) 可溶性載質(zhì)轉(zhuǎn)運(yùn)蛋白SLC24A5(抗原)
solute carrier family 1 溶質(zhì)攜帶物家族-1(抗原)
SLC6A4 (solute carrier family 6) 鉀依賴鈉鈣交換蛋白6(抗原)
Survivin 細(xì)胞凋亡抑制因子的一種(抗原)
BIRC5/Survivin variant 細(xì)胞凋亡抑制因子4(抗原)
SSA/RO (ribonucleoprotein plex,RNP) 核糖核蛋白抗原SSA(抗原)
SSB/La(Sjogren syndrome type B antigen)(抗原) 抗干燥癥SSB/La蛋白抗體
Smad2(Mothers against decapentaplegic homolog 2) Smad 2/3(抗原)
SPAG5/map126 (sperm associated antigen 5) 抗精子相關(guān)抗原5(抗原)
SP-A (pulmonary surfactant-associated glycoprotein A) 肺表面活性蛋白A(抗原)
SP-A (pulmonary surfactant-associated glycoprotein A) 肺表面活性蛋白A(抗原)
SYVN1(synovial apoptosis inhibitor 1) 滑膜細(xì)胞凋亡抑制物1(抗體)
SYK (Spleen tyrosine kinase) 非受體型酪氨酸激酶(抗原)
Tau protein 微管相關(guān)蛋白(抗原)
TGF-βR 1/ALK-5(TGF-beta receptor type I) 轉(zhuǎn)移生長因子–β受體 1(抗原)
Thy-1/CD90 CD90(抗原)
Thioster-containing protein 1 硫酯包含蛋白-1(抗原)
TIGAR humen (TP53- induced glycolysis and apoptosis-regulator ) P53誘導(dǎo)糖酵解和凋亡調(diào)節(jié)因子蛋白(抗原)
TIMP-4(Tissue Inhibitor of Metalloproteinase-4) 金屬蛋白酶組織抑制因子-4(抗原)
TPO(Thyroid Peroxidase) 甲狀腺過氧化物酶(抗原)
TSHR (Thyroid stimulating hormone receptor) 促甲狀腺素受體(抗原)
TSLP(Thymic stromal lymphopoietin isoform 1) 淋巴細(xì)胞生成素-1(抗原)
TTF-2(thyroid transcription factor 2;forkhead box E1) 甲狀腺轉(zhuǎn)錄因子-2(抗原)
Tyrosinase 酪氨酸酶(多肽抗原)
OAT4L又稱URAT1 (Urate Transporter 1) 尿酸鹽重吸收轉(zhuǎn)運(yùn)子1 (抗原)
VAP1(vascular adhesion protein 1) 血管粘附蛋白1(多肽抗原)
VCAM-1(Vascuolar cell adhesion molecule 1)CD106 血管內(nèi)皮細(xì)胞粘附分子(抗原)
Vimentin 波形蛋白(抗原)
VSIG4 (V-set and immunoglobulin domain-containing protein 4) VSIG4 T淋巴細(xì)胞負(fù)調(diào)節(jié)蛋白之一(抗原)
WNK4(Ser/Thr-protein kinase WNK4; protein kinase with no lysine 4; protein kinase, lysine –dficient 4) 一種新的絲氨酸/蘇氨酸激酶家族成員的基因WNK4(多肽抗原)
ZNF300 (zinc finger protein ZNF300)- middle 鋅指蛋白300(抗原)
ZNF268 (zinc finger protein ZNF268)- N-terminal (1a) 鋅指脂蛋白-1a(抗原)
ZNF268 (zinc finger protein ZNF268)- middle (1b) 鋅指脂蛋白-1b(抗原)
ZNF268 (zinc finger protein ZNF268)- middle (1c) 人鋅指脂蛋白-1c(抗原)
IFN-γ(Interferon γ)Ag-mouse、rat 干擾素-γ多肽抗原(大、小鼠)
IFN-γ(Interferon γ)Ag-Human 干擾素-γ多肽抗原(人)
IFN-β (Interferon β)Anti-mouse,rat 干擾素-β抗原
IFN-β (Interferon β)Anti-human 干擾素-β抗原
PSCA (Prostate Stem Cell Antigen) 前列腺干細(xì)胞抗原
sIgA 齊一生物供應(yīng)分泌型IgA(抗原)
化合物與牛血清白蛋白R(shí)CA偶聯(lián)抗原 化合物與血藍(lán)蛋白KLH偶聯(lián)抗原
Melamine/KLH 三聚氰胺與血藍(lán)蛋白偶聯(lián)物
Melamine/RCA 三聚氰胺與牛血清白蛋白偶聯(lián)物
Melamine/OVA(ovalbumin) 三聚氰胺與雞卵白蛋白偶聯(lián)物